The hottest Sequencing Substack posts right now

And their main takeaways
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Top Science Topics

Roche Nanopore Expectation Management

ASeq Newsletter • 29 implied HN points • 23 Jan 25
🔬 Science Genetics Sequencing Biotechnology Research Innovation
  1. The Roche Nanopore sequencer is expected to generate useful sequence data, showcasing its potential in the market. This could help solidify nanopores as a valuable detection technology.
  2. Accuracy is important for applications, but Roche is likely to compete on cost, which could make their technology appealing for various uses. Their position is good for future improvements.
  3. Roche's innovations may lead to significant competition for existing sequencing methods, especially Illumina. The expectations suggest that this could reshape the sequencing landscape.

How To Respond To Roche

ASeq Newsletter • 21 implied HN points • 28 Jan 25
🕹 Technology Sequencing Biotech Innovation Industry Research
  1. Roche's upcoming announcement could greatly change the DNA sequencing industry by introducing low-cost, high-output nanopore sequencing technology.
  2. Even if the quality of Roche's sequencing isn't the best now, the high throughput they promise means they could outpace current methods and become competitive.
  3. Current sequencing companies need to rethink their strategies quickly to stay relevant or they might risk being outmatched and going extinct in this evolving market.

Year In Review - Everything Else!

ASeq Newsletter • 14 implied HN points • 20 Dec 24
🔬 Science Genomics Sequencing Biotechnology
  1. Clinical sequencing is becoming a major part of the medical field, especially in areas like oncology and genetic disease testing. However, many clinicians still find it challenging to use these new testing methods.
  2. Protein sequencing is an emerging area with potential advancements on the horizon, particularly with new technologies like Nanopore. It's an exciting field that many are watching closely.
  3. Single cell analysis remains a hot topic, but companies like 10X Genomics are facing challenges with growth and patent issues. There are still many experiments and innovations happening in this space.
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Mapping your company's year: how to sequence the big processes

Lessons • 255 implied HN points • 07 Apr 23
💼 Business Planning Budgeting Performance reviews Sequencing
  1. Sequencing big projects in your company efficiently can save time and streamline processes.
  2. Key company-wide activities to schedule include board meetings, business reviews, budgeting, and performance reviews.
  3. Being thoughtful about the timing of important processes like performance reviews can impact company productivity.

Year In Review - Long Reads

ASeq Newsletter • 7 implied HN points • 18 Dec 24
🕹 Technology Genomics Sequencing Biotechnology Medical Research Health tech
  1. PacBio faced challenges in the stock market but still aims to provide high-quality clinical genomic sequencing. They are seen as a strong competitor in the long-read space despite financial struggles.
  2. Oxford Nanopore has been active in raising funds but has dealt with internal issues and departures of significant team members. Profitability remains an elusive goal for both them and PacBio.
  3. New competitors are emerging in the long-read sequencing market, including Roche and BGI, who are exploring nanopore sequencing technology. This could shake up the landscape for current players.

4 Reads Per Cluster! Simultaneously Paired End-Sequencing!

ASeq Newsletter • 36 implied HN points • 31 Jan 24
🔬 Science Genetics Bioinformatics Sequencing Biotechnology
  1. Illumina has a method to potentially double their instruments' throughput, but it may come with a slight decrease in accuracy.
  2. By simultaneously reading both the forward and reverse strands, Illumina can achieve four reads per cluster, doubling the throughput.
  3. Implementing the simultaneous paired-end sequencing approach may be challenging without sacrificing accuracy, but it opens up opportunities for increased throughput in the future.

Illumina Reagent Volumes And Recipes

ASeq Newsletter • 58 implied HN points • 27 Aug 23
🕹 Technology Research Sequencing Recipes
  1. Illumina does not provide public disclosure on reagent volumes, but you can measure them from kits.
  2. Recipe files for different Illumina sequencing platforms are available online to guide reagent amounts for the flowcell.
  3. A Google doc has extracted reagent steps for a deblock/incorporation cycle from various Illumina recipe files.

Comments On "An Illumina Sequencer in 2000"

ASeq Newsletter • 21 implied HN points • 21 Feb 24
🕹 Technology Innovation Biotech Startups Sequencing Acquisition
  1. The acquisition of Solexa by Illumina involved strategic financial decisions and a combination of technology strengths to create a successful sequencing platform.
  2. There were missed opportunities and challenges due to financial constraints and differing viewpoints among key players in the sequencing technology industry.
  3. The early 2000s marked a period of significant innovation and the emergence of next-generation sequencing technologies, with various companies maneuvering to establish themselves in the market.

What The H*ll Is PPMSeq?

ASeq Newsletter • 21 implied HN points • 17 Feb 24
🔬 Science Biotechnology Genomics Sequencing Bioinformatics
  1. PPMSeq is a duplex sequencing technique that ensures high accuracy reads by reading both forward and reverse strands of a double-stranded sequence.
  2. Ultima's PPMSeq works by amplifying both strands on the same bead and utilizing a tag to identify their presence, with errors causing radical dephasing which is taken care of by downstream software.
  3. Approaches to implementing PPMSeq on Ultima's platform involve reading both strands in both directions simultaneously or keeping the product on beads in the same orientation, each with its own challenges and considerations.

PacBio Market Cap <$1B

ASeq Newsletter • 7 implied HN points • 18 Mar 24
🕹 Technology Market Analysis Genomics Competitors Sequencing
  1. PacBio's market cap drops below $1B, making it potentially attractive for acquisition.
  2. Despite some positives like new instruments and competitive specs, PacBio faces challenges in gaining traction for long-read sequencing in clinical settings.
  3. The majority of PacBio's IP expires in 2029, raising concerns that competitors could enter the market in the near future.

Maybe Nanopore Protein Sequencing Is Easier Than I Thought?... Maybe Not?...

ASeq Newsletter • 7 implied HN points • 12 Mar 24
🔬 Science Proteomics Sequencing
  1. Protein sequencing can potentially be easier than expected with nanopore technology, allowing for detection of PTMs and obtaining unique fingerprints from proteins.
  2. Proteomics differs from DNA sequencing in that it allows for estimating protein abundance and identifying PTMs in samples, possibly through aligning multiple protein traces.
  3. Challenges in proteomics applications with nanopore platforms include achieving the necessary dynamic range for accurate measurements, which may require advancements in technology.

All Aboard The NanoTrain! Chu Chu!

ASeq Newsletter • 0 implied HN points • 31 Oct 24
🔬 Science Biotechnology Genetics Sequencing Research Technology
  1. Universal Sequencing Technology is gaining attention again after being quiet for a while. People are curious about their progress and what they are focusing on now.
  2. They seem to have shifted their focus from single molecule sequencing to developing various sample preparation kits. This shows they are adapting to changes in the field.
  3. This update may be particularly interesting for those who follow advancements in sequencing technology and want to know about new tools or methods in the industry.