The hottest Sequencing Substack posts right now

And their main takeaways
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Top Science Topics

4 Reads Per Cluster! Simultaneously Paired End-Sequencing!

ASeq Newsletter • 36 implied HN points • 31 Jan 24
🔬 Science Genetics Bioinformatics Sequencing Biotechnology
  1. Illumina has a method to potentially double their instruments' throughput, but it may come with a slight decrease in accuracy.
  2. By simultaneously reading both the forward and reverse strands, Illumina can achieve four reads per cluster, doubling the throughput.
  3. Implementing the simultaneous paired-end sequencing approach may be challenging without sacrificing accuracy, but it opens up opportunities for increased throughput in the future.

Comments On "An Illumina Sequencer in 2000"

ASeq Newsletter • 21 implied HN points • 21 Feb 24
🕹 Technology Innovation Biotech Startups Sequencing Acquisition
  1. The acquisition of Solexa by Illumina involved strategic financial decisions and a combination of technology strengths to create a successful sequencing platform.
  2. There were missed opportunities and challenges due to financial constraints and differing viewpoints among key players in the sequencing technology industry.
  3. The early 2000s marked a period of significant innovation and the emergence of next-generation sequencing technologies, with various companies maneuvering to establish themselves in the market.
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What The H*ll Is PPMSeq?

ASeq Newsletter • 21 implied HN points • 17 Feb 24
🔬 Science Biotechnology Genomics Sequencing Bioinformatics
  1. PPMSeq is a duplex sequencing technique that ensures high accuracy reads by reading both forward and reverse strands of a double-stranded sequence.
  2. Ultima's PPMSeq works by amplifying both strands on the same bead and utilizing a tag to identify their presence, with errors causing radical dephasing which is taken care of by downstream software.
  3. Approaches to implementing PPMSeq on Ultima's platform involve reading both strands in both directions simultaneously or keeping the product on beads in the same orientation, each with its own challenges and considerations.

PacBio Market Cap <$1B

ASeq Newsletter • 7 implied HN points • 18 Mar 24
🕹 Technology Market Analysis Genomics Competitors Sequencing
  1. PacBio's market cap drops below $1B, making it potentially attractive for acquisition.
  2. Despite some positives like new instruments and competitive specs, PacBio faces challenges in gaining traction for long-read sequencing in clinical settings.
  3. The majority of PacBio's IP expires in 2029, raising concerns that competitors could enter the market in the near future.

Maybe Nanopore Protein Sequencing Is Easier Than I Thought?... Maybe Not?...

ASeq Newsletter • 7 implied HN points • 12 Mar 24
🔬 Science Sequencing
  1. Protein sequencing can potentially be easier than expected with nanopore technology, allowing for detection of PTMs and obtaining unique fingerprints from proteins.
  2. Proteomics differs from DNA sequencing in that it allows for estimating protein abundance and identifying PTMs in samples, possibly through aligning multiple protein traces.
  3. Challenges in proteomics applications with nanopore platforms include achieving the necessary dynamic range for accurate measurements, which may require advancements in technology.

Mapping your company's year: how to sequence the big processes

Lessons • 255 implied HN points • 07 Apr 23
💼 Business Planning Budgeting Performance reviews Sequencing
  1. Sequencing big projects in your company efficiently can save time and streamline processes.
  2. Key company-wide activities to schedule include board meetings, business reviews, budgeting, and performance reviews.
  3. Being thoughtful about the timing of important processes like performance reviews can impact company productivity.

Illumina Reagent Volumes And Recipes

ASeq Newsletter • 58 implied HN points • 27 Aug 23
🕹 Technology Research Sequencing Recipes
  1. Illumina does not provide public disclosure on reagent volumes, but you can measure them from kits.
  2. Recipe files for different Illumina sequencing platforms are available online to guide reagent amounts for the flowcell.
  3. A Google doc has extracted reagent steps for a deblock/incorporation cycle from various Illumina recipe files.

PacBio Acquires Apton!

ASeq Newsletter • 58 implied HN points • 04 Aug 23
🕹 Technology Biotech Acquisition Sequencing Innovation
  1. PacBio is acquiring Apton BioSystems for approximately $110M to enhance their sequencing technology.
  2. The acquisition of Apton seems strategic for PacBio to develop a competitive high-throughput sequencing instrument.
  3. PacBio's new instrument lineup includes a range of sequencers from mid-range short reads to high-throughput long reads.

Thoughts On Pacific Biosciences

ASeq Newsletter • 29 implied HN points • 14 Sep 23
🔬 Science Sequencing Accuracy
  1. PacBio's raw accuracy has only slightly improved over 4 generations of instruments.
  2. Fundamental issues, like polymerase kinetics, may be limiting the accuracy of PacBio sequencing.
  3. PacBio has prioritized longer read lengths over accuracy, using cyclic consensus sequencing for improved results.