ASeq Newsletter • 21 implied HN points • 17 Mar 26
- They’re building a high-plex PCR approach that runs on standard qPCR and dPCR machines, already showing up to 15 targets in dPCR and a 7‑target qPCR prototype, with aims of roughly 50 and 40 targets respectively.
- The key idea is to move fluorescent signal generation out of the genomic amplification and into a parallel isothermal secondary reaction; probe cleavage during PCR produces a cleaved tail that triggers separate signal‑generating chemistries, effectively acting like a barcode.
- By decoupling signal chemistry from amplification and pooling fluorophores separately, the method could let developers multiplex many targets in a single reaction without needing specialized instrumentation.